LC3A-mediated autophagy regulates lung cancer cell plasticity

Chia-Cheng Miao; Wen Hwang; Ling-Yi Chu; Li-Hao Yang; Cam-Thu Ha; Pei-Yu Chen; Ming-Han Kuo; Sheng-Chieh Lin; Ya-Yu Yang; Shuang-En Chuang; Chia-Cherng Yu; Shien-Tung Pan; Mou-Chieh Kao; Chuang-Rung Chang; Yu-Ting Chou

doi:10.1080/15548627.2021.1964224

LC3A-mediated autophagy regulates lung cancer cell plasticity

Autophagy. 2022 Apr;18(4):921-934. doi: 10.1080/15548627.2021.1964224. Epub 2021 Sep 1.

Authors

Chia-Cheng Miao^{1

2}, Wen Hwang^{1

2}, Ling-Yi Chu¹, Li-Hao Yang¹, Cam-Thu Ha¹, Pei-Yu Chen¹, Ming-Han Kuo¹, Sheng-Chieh Lin^{1

3}, Ya-Yu Yang⁴, Shuang-En Chuang⁴, Chia-Cherng Yu⁵, Shien-Tung Pan⁶, Mou-Chieh Kao⁷, Chuang-Rung Chang¹, Yu-Ting Chou¹

Affiliations

¹ Institute Of Biotechnology, National Tsing Hua University, Hsinchu, Taiwan (R.O.C.).
² Co-first Authors.
³ Graduate Institute Of Integrated Medicine, China Medical University, Taichung, Taiwan (R.O.C.).
⁴ National Institute Of Cancer Research, National Health Research Institutes, Miaoli, Taiwan (R.O.C.).
⁵ Department Of Medical Research, National Taiwan University Hospital, Taipei, Taiwan (R.O.C.).
⁶ Department Of Pathology, China Medical University Hsinchu Hospital, Hsinchu County, Taiwan (R.O.C.).
⁷ Institute Of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan (R.O.C.).

Abstract

ATG14: autophagy related 14; CDH2: cadherin 2; ChIP-qPCR: chromatin immunoprecipitation quantitative polymerase chain reaction; CQ: chloroquine; ECAR: extracellular acidification rate; EMT: epithelial-mesenchymal transition; EPCAM: epithelial cell adhesion molecule; MAP1LC3A/LC3A: microtubule associated protein 1 light chain 3 alpha; MAP1LC3B/LC3B: microtubule associated protein 1 light chain 3 beta; MAP1LC3C/LC3C: microtubule associated protein 1 light chain 3 gamma; NDUFV2: NADH:ubiquinone oxidoreductase core subunit V2; OCR: oxygen consumption rate; ROS: reactive oxygen species; RT-qPCR: reverse-transcriptase quantitative polymerase chain reaction; SC: scrambled control; shRNA: short hairpin RNA; SNAI2: snail family transcriptional repressor 2; SOX2: SRY-box transcription factor 2; SQSTM1/p62: sequestosome 1; TGFB/TGF-β: transforming growth factor beta; TOMM20: translocase of outer mitochondrial membrane 20; ZEB1: zinc finger E-box binding homeobox 1.

Keywords: Autophagy; LC3A; SOX2; cancer cell plasticity; lung cancer; mitochondria dynamics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autophagy* / physiology
Cell Plasticity
Humans
Lung Neoplasms*
Microtubule-Associated Proteins / metabolism
Reactive Oxygen Species / metabolism

Substances

Microtubule-Associated Proteins
Reactive Oxygen Species

Grants and funding

This work was supported by National Tsing Hua University and Ministry of Science and Technology [106-2320-B-007-005-MY3 and 109-2320-B-007-003-MY3], Executive Yuan, Taiwan (R.O.C.).