PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava

Siji Kavil; Gerald Otti; Sophie Bouvaine; Andrew Armitage; Midatharahally N Maruthi

doi:10.1186/s12985-021-01649-2

PAL1 gene of the phenylpropanoid pathway increases resistance to the Cassava brown streak virus in cassava

Virol J. 2021 Sep 9;18(1):184. doi: 10.1186/s12985-021-01649-2.

Authors

Siji Kavil¹, Gerald Otti^{1

2}, Sophie Bouvaine¹, Andrew Armitage¹, Midatharahally N Maruthi³

Affiliations

¹ Agriculture, Health and Environment Department, Natural Resources Institute, University of Greenwich, Medway campus, Chatham, Kent, ME4 4TB, UK.
² Computational and Analytical Sciences, Rothamsted Research, Harpenden, AL5 2JQ, UK.
³ Agriculture, Health and Environment Department, Natural Resources Institute, University of Greenwich, Medway campus, Chatham, Kent, ME4 4TB, UK. M.N.Maruthi@greenwich.ac.uk.

Abstract

Background: The phenylalanine ammonia lyase genes play crucial role in plant response to biotic and abiotic stresses. In this study, we characterized the role of PAL genes in increasing resistance to the Cassava brown streak virus that causes the economically important cassava brown streak disease (CBSD) on cassava in Africa.

Methods: The whole transcriptomes of eight cassava varieties differing in resistance to CBSD were obtained at 1, 5 and 8 weeks after CBSV infection.

Results: Analysis of RNA-Seq data identified the overexpression of PAL1, PAL2, cinnamic acid and two chalcone synthase genes in CBSD-resistant cassava varieties, which was subsequently confirmed by RT-qPCR. The exogenous application of Acibenzolar-S-Methyl induced PAL1 gene expression to enhance resistance in the susceptible var. Kalawe. In contrast, the silencing of PAL1 by RNA interference led to increased susceptibility of the resistant var. Kaleso to CBSD.

Conclusions: PAL1 gene of the phenylpropanoid pathway has a major role in inducing resistance to CBSD in cassava plants and its early induction is key for CBSD resistance.

Keywords: CBSD; Cassava; Induction; PAL1; Resistance.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disease Resistance* / genetics
Manihot* / genetics
Manihot* / virology
Plant Diseases* / genetics
Plant Diseases* / virology
Potyviridae* / pathogenicity

Supplementary concepts

Cassava brown streak virus