Biodegradable mesoporous manganese carbonate nanocomposites for LED light-driven cancer therapy via enhancing photodynamic therapy and attenuating survivin expression

Lihua Li; Lingling Chen; Ling Huang; Xiangling Ye; Zefeng Lin; Xiaoming Wei; Xianfeng Yang; Zhongmin Yang

doi:10.1186/s12951-021-01057-2

Biodegradable mesoporous manganese carbonate nanocomposites for LED light-driven cancer therapy via enhancing photodynamic therapy and attenuating survivin expression

J Nanobiotechnology. 2021 Oct 9;19(1):310. doi: 10.1186/s12951-021-01057-2.

Authors

Lihua Li¹, Lingling Chen², Ling Huang³, Xiangling Ye⁴, Zefeng Lin², Xiaoming Wei³, Xianfeng Yang⁵, Zhongmin Yang⁶

Affiliations

¹ The State Key Laboratory of Luminescent Materials and Devices; Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, Analytical and Testing Center, South China University of Technology, Guangzhou, 510640, Guangdong, China. lilihua@scut.edu.cn.
² Guangdong Key Lab of Orthopedic Technology and Implant, General Hospital of Southern Theater Command of PLA, The First School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, China.
³ The State Key Laboratory of Luminescent Materials and Devices; Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, Analytical and Testing Center, South China University of Technology, Guangzhou, 510640, Guangdong, China.
⁴ The Fifth Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, 510095, China.
⁵ The State Key Laboratory of Luminescent Materials and Devices; Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, Analytical and Testing Center, South China University of Technology, Guangzhou, 510640, Guangdong, China. czxfyang@scut.edu.cn.
⁶ The State Key Laboratory of Luminescent Materials and Devices; Guangdong Provincial Key Laboratory of Fiber Laser Materials and Applied Techniques, Analytical and Testing Center, South China University of Technology, Guangzhou, 510640, Guangdong, China. yangzm@scut.edu.cn.

Abstract

Triple-negative breast cancer (TNBC) is one of the most daunting diseases, low toxicity and efficient approaches are in urgent demand. Herein, we developed degradable mesoporous manganese carbonate nanocubes (MnCO₃ NCs), incorporated with survivin shRNA-expressing plasmid DNA (iSur-pDNA) and riboflavin (Rf), namely MRp NCs, for synergistic TNBC therapy. The MnCO₃, itself, could generate O₂ and CO₂ under H₂O₂ and thus relieve the hypoxia and acidic tumor microenvironment (TME). Furthermore, the MnCO₃ NCs exhibited high Rf loading capacity and iSur-pDNA delivery ability after polyethyleneimine modification. Specifically, MRp NCs decompose in TME, meanwhile they deprived the endogenous expression of survivin gene and significantly amplified the generation of reactive oxygen species after exposure to LED light, resulting in serious tumor destruction. The multifunctional MRp NCs with LED light-driven characters are able to provide a high efficiency, low toxicity and promising strategy for TNBC therapy.

Keywords: LED light responsive; Mesoporous MnCO3 nanocubes; O2 and CO2 release; Reactive oxygen species; Triple negative breast cancer.

MeSH terms

Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology
Carbonates* / chemistry
Carbonates* / pharmacology
Cell Line, Tumor
Cell Survival / drug effects
Female
Manganese* / chemistry
Manganese* / pharmacology
Mice
Mice, Nude
Nanocomposites* / chemistry
Nanocomposites* / toxicity
Photochemotherapy*
Photosensitizing Agents / chemistry
Photosensitizing Agents / pharmacology
Reactive Oxygen Species / metabolism
Survivin* / genetics
Survivin* / metabolism
Triple Negative Breast Neoplasms* / metabolism
Triple Negative Breast Neoplasms* / pathology
Tumor Microenvironment / drug effects

Substances

Antineoplastic Agents
Carbonates
Photosensitizing Agents
Reactive Oxygen Species
Survivin
Manganese
manganese carbonate

Abstract

MeSH terms

Substances

Grants and funding