The simultaneous incorporation of distinct noncanonical amino acids into different proteins within eukaryotic cells remains challenging. This new study by Reinkemeierand Lemke demonstrates how 2D phase separation can be used to engineer spatially separated organelles. These film-like organelles translate proteins independently from each other and the canonical genetic code.
Keywords: 2D phase separation; enzyme engineering; genetic code expansion; noncanonical amino acid; synthetic biology; synthetic organelle.
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