Quantitative Mass Spectrometry Imaging with Liquid Microjunction Surface Sampling

Qian Wu; Hongmei Lu

doi:10.1007/978-1-0716-2030-4_13

Quantitative Mass Spectrometry Imaging with Liquid Microjunction Surface Sampling

Methods Mol Biol. 2022:2437:181-194. doi: 10.1007/978-1-0716-2030-4_13.

Authors

Qian Wu¹, Hongmei Lu²

Affiliations

¹ College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, P. R. China.
² College of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, P. R. China. hongmeilu@csu.edu.cn.

PMID: 34902149
DOI: 10.1007/978-1-0716-2030-4_13

Abstract

Mass spectrometry imaging (MSI) could provide chemical spatial distribution within a diverse range of samples, but absolute quantitation with those techniques is still challenging. Recent years, ambient liquid extraction-based MSI techniques, such as liquid microjunction surface sampling (LMJSS), have been largely developed and were found to be favorable to quantitation by directly doping standards in the extraction solvent. Here, we describe the detailed experimental protocols and the data processing methods for quantitative MSI with LMJSS. The new methods could have absolute quantitative MSI of both endogenous lipids and small metabolites from tissue samples.

Keywords: Calibration methods; Lipidomics; Liquid microjunction surface sampling; Metabolomics; Quantitative mass spectrometry imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Diagnostic Imaging
Lipids
Mass Spectrometry*
Solvents

Substances

Lipids
Solvents