Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces

Varvara Chalmantzi; Chara Simitzi; Angelos Papadopoulos; Eleni Bagli; Carol Murphy; Emmanuel Stratakis; Theodore Fotsis

doi:10.1007/7651_2021_428

Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces

Methods Mol Biol. 2022:2454:49-59. doi: 10.1007/7651_2021_428.

Authors

Varvara Chalmantzi^{1

2}, Chara Simitzi^{3

4}, Angelos Papadopoulos^{1

2}, Eleni Bagli², Carol Murphy^{1

2}, Emmanuel Stratakis^{5

6}, Theodore Fotsis^{7

8}

Affiliations

¹ School of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham, UK.
² Department of Biomedical Research, Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology-Hellas, Ioannina, Greece.
³ Institute of Electronic Structure and Laser (IESL), Foundation of Research and Technology Hellas (FORTH), Vassilika Vouton, Heraklion, Greece.
⁴ Institute of Materials Discovery, University College London, London, UK.
⁵ Institute of Electronic Structure and Laser (IESL), Foundation of Research and Technology Hellas (FORTH), Vassilika Vouton, Heraklion, Greece. stratak@iesl.forth.gr.
⁶ Materials Science and Technology Department, University of Crete, Vassilika Voutes, Heraklion, Greece. stratak@iesl.forth.gr.
⁷ Department of Biomedical Research, Institute of Molecular Biology and Biotechnology, Foundation of Research and Technology-Hellas, Ioannina, Greece. thfotsis@uoi.gr.
⁸ Laboratory of Biological Chemistry, Medical School, University of Ioannina, Ioannina, Greece. thfotsis@uoi.gr.

PMID: 34907510
DOI: 10.1007/7651_2021_428

Abstract

Human pluripotent stem cell culture conditions are constantly being optimized, thus providing insight to the environmental cues that affect cell choices. A wide variety of media, coating materials, and substrates is now available for use, serving different scientific needs. Factors such as material stiffness, roughness, and topography are being recognized to contribute or even direct the acquisition of specific phenotypes. Here, we describe the use of patterned silicon substrates coated with Matrigel for the propagation and differentiation of human pluripotent stem cells.

Keywords: BRACHYURY; Immunofluorescence; Laser manufacturing; NANOG; Silicon substrates; Surface micropatterning; Topography; hPSCs.

MeSH terms

Cell Differentiation / genetics
Humans
Pluripotent Stem Cells*
Silicon*

Substances

Silicon