Fluorescence-Based Biosensors for the Detection of the Unfolded Protein Response

Heinrich Kroukamp; Kai Peng; Ian T Paulsen; Riaan den Haan

doi:10.1007/978-1-0716-1732-8_2

Fluorescence-Based Biosensors for the Detection of the Unfolded Protein Response

Methods Mol Biol. 2022:2378:19-30. doi: 10.1007/978-1-0716-1732-8_2.

Authors

Heinrich Kroukamp^{1

2}, Kai Peng¹, Ian T Paulsen^{1

2}, Riaan den Haan³

Affiliations

¹ Department of Molecular Sciences, ARC Centre of Excellence in Synthetic Biology, Macquarie University, Sydney, NSW, Australia.
² Biomolecular Discovery and Design Research Centre, Macquarie University, Sydney, NSW, Australia.
³ Department of Biotechnology, University of the Western Cape, Bellville, South Africa. rdenhaan@uwc.ac.za.

PMID: 34985691
DOI: 10.1007/978-1-0716-1732-8_2

Abstract

The unfolded protein response (UPR) is a highly conserved protein quality control mechanism of eukaryotic cells. Aberrations in this response have been linked to several human diseases, including retinitis pigmentosa and several cancers, and have been shown to have a drastic impact on recombinant protein yields in fungal, insect, and mammalian cell lines. Here, we describe the use of in vivo biosensors to measure and characterize this dynamic cellular response, specifically for detecting the UPR induced by protein overproduction stress in the model cell factory Saccharomyces cerevisiae.

Keywords: Biosensors; Protein folding capacity; Saccharomyces cerevisiae; Unfolded protein response.

MeSH terms

Animals
Biosensing Techniques*
Endoplasmic Reticulum / metabolism
Humans
Mammals / metabolism
Protein Folding
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins* / metabolism
Unfolded Protein Response

Substances

Saccharomyces cerevisiae Proteins