A hydrophilic interaction liquid chromatography - Tandem mass spectrometry method for the determination of phenylephrine in dried blood spots from preterm infants

E Boutou; C Virgiliou; A K Seliniotaki; M Lithoxopoulou; A Mataftsi; N Ziakas; E Diamanti; N Raikos; H Gika

doi:10.1016/j.jchromb.2021.123084

A hydrophilic interaction liquid chromatography - Tandem mass spectrometry method for the determination of phenylephrine in dried blood spots from preterm infants

J Chromatogr B Analyt Technol Biomed Life Sci. 2022 Feb 1:1190:123084. doi: 10.1016/j.jchromb.2021.123084. Epub 2022 Jan 6.

Authors

E Boutou¹, C Virgiliou¹, A K Seliniotaki², M Lithoxopoulou³, A Mataftsi², N Ziakas², E Diamanti³, N Raikos⁴, H Gika⁵

Affiliations

¹ Department of Chemistry, Aristotle University of Thessaloniki, Greece.
² 2nd Department of Ophthalmology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
³ 2nd Department of Neonatology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Thessaloniki, Greece.
⁴ Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece.
⁵ Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Greece. Electronic address: gkikae@auth.gr.

PMID: 35032892
DOI: 10.1016/j.jchromb.2021.123084

Abstract

A sensitive and accurate hydrophilic interaction liquid chromatography - tandem mass spectrometry method (HILIC-MS/MS) was developed and validated for the determination of phenylephrine concentration in Dried Blood Spot (DBS) samples from preterm infants, after ocular administration of an ophthalmic solution with phenylephrine. Sample preparation involved the extraction of the analyte from an 85 μL DBS sample with methanol - acetonitrile (50:50, v/v). Chromatographic separation was achieved on an ACQUITY UPLC BEH AMIDE column, under isocratic conditions within a 5 min run. Detection was achieved with a triple quadrupole MS applying electrospray ionization in positive mode. The method was fully validated and proved precise and accurate with in a linear range of 0.59-3.53 ng/ml in blood. The method was developed to provide insights on the level of exposure of infant population to phenylephrine after ocular administration.

Keywords: DBS; Drug; HILIC; Pharmaceutical; UPLC-MS/MS.

Publication types

Evaluation Study

MeSH terms

Chromatography, High Pressure Liquid / methods*
Dried Blood Spot Testing / methods*
Eye Diseases, Hereditary / blood
Eye Diseases, Hereditary / diagnosis*
Female
Humans
Infant
Infant, Newborn
Infant, Newborn, Diseases / blood
Infant, Newborn, Diseases / diagnosis*
Infant, Premature / blood*
Male
Mydriasis / blood
Mydriasis / diagnosis*
Mydriatics / administration & dosage
Mydriatics / blood*
Ophthalmic Solutions
Phenylephrine / administration & dosage
Phenylephrine / blood*
Tandem Mass Spectrometry / methods*

Substances

Mydriatics
Ophthalmic Solutions
Phenylephrine

Supplementary concepts

Mydriasis, Congenital