Rapid Nucleic Acid Reaction Circuits for Point-of-care Diagnosis of Diseases

Ezry Santiago-McRae; Sung Won Oh; Anthony Monte Carlo; Omri Bar; Emily Guan; Doris Zheng; Catherine Grgicak; Jinglin Fu

doi:10.2174/1570163819666220207114148

Rapid Nucleic Acid Reaction Circuits for Point-of-care Diagnosis of Diseases

Curr Top Med Chem. 2022;22(8):686-698. doi: 10.2174/1570163819666220207114148.

Authors

Ezry Santiago-McRae¹, Sung Won Oh^{1

2}, Anthony Monte Carlo², Omri Bar², Emily Guan³, Doris Zheng², Catherine Grgicak^{1

2}, Jinglin Fu^{1

2}

Affiliations

¹ Center for Computational and Integrative Biology, The State University of New Jersey, Rutgers, USA.
² Department of Chemistry, Rutgers University-Camden, Camden, NJ 08102, USA.
³ High Technology High School, Lincroft, NJ 07738, USA.

PMID: 35139798
DOI: 10.2174/1570163819666220207114148

Abstract

An urgent need exists for a rapid, cost-effective, facile, and reliable nucleic acid assay for mass screening to control and prevent the spread of emerging pandemic diseases. This urgent need is not fully met by current diagnostic tools. In this review, we summarize the current state-of-the-art research in novel nucleic acid amplification and detection that could be applied to point-of-care (POC) diagnosis and mass screening of diseases. The critical technological breakthroughs will be discussed for their advantages and disadvantages. Finally, we will discuss the future challenges of developing nucleic acid-based POC diagnosis.

Keywords: CRISPR-Cas; Diseases; Isothermal amplification; Nucleic acid assay; Nucleic acid circuits; Point-of-care.

Publication types

Review

MeSH terms

Nucleic Acid Amplification Techniques
Nucleic Acids*
Pandemics
Point-of-Care Systems

Substances

Nucleic Acids

Abstract

Publication types

MeSH terms

Substances

Grants and funding