Caenimonas aquaedulcis sp. nov., Isolated from Freshwater of Daechung Reservoir during Microcystis Bloom

Ve Van Le; So-Ra Ko; Sang-Ah Lee; Mingyeong Kang; Hee-Mock Oh; Chi-Yong Ahn

doi:10.4014/jmb.2201.01023

Caenimonas aquaedulcis sp. nov., Isolated from Freshwater of Daechung Reservoir during Microcystis Bloom

J Microbiol Biotechnol. 2022 May 28;32(5):575-581. doi: 10.4014/jmb.2201.01023.

Authors

Ve Van Le^{1

2}, So-Ra Ko¹, Sang-Ah Lee³, Mingyeong Kang^{1

2}, Hee-Mock Oh^{1

2}, Chi-Yong Ahn^{1

2}

Affiliations

¹ Cell factory Research Centre, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
² Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea.
³ Environmental Safety Groups, Korea Institute of Science and Technology (KIST) Europe, Saarbrücken 66123, Germany.

Abstract

A Gram-stain-negative, white-coloured, and rod-shaped bacterium, strain DR4-4^T, was isolated from Daechung Reservoir, Republic of Korea, during Microcystis bloom. Strain DR4-4^T was most closely related to Caenimonas terrae SGM1-15^T and Caenimonas koreensis EMB320^T with 98.1% 16S rRNA gene sequence similarities. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain DR4-4^T and closely related type strains were below 79.46% and 22.30%, respectively. The genomic DNA G+C content was 67.5%. The major cellular fatty acids (≥10% of the total) were identified as C_16:0, cyclo C_17:0, summed feature 3 (C_16:1ω7c and/or C_16:1ω6c), and summed feature 8 (C_18:1ω7c and/or C_18:1ω6c). Strain DR4-4^T possessed phosphatidylethanolamine, diphosphatidylglycerol, and phosphatidylglycerol as the main polar lipids and Q-8 as the respiratory quinone. The polyamine profile was composed of putrescine, cadaverine, and spermidine. The results of polyphasic characterization indicated that the isolated strain DR4-4^T represents a novel species within the genus Caenimonas, for which the name Caenimonas aquaedulcis sp. nov. is proposed. The type strain is DR4-4^T (=KCTC 82470^T =JCM 34453^T).

Keywords: Caenimonas aquaedulcis; Microcystis aeruginosa; freshwater; polyphasic characterization.

MeSH terms

Bacterial Typing Techniques
DNA, Bacterial / genetics
Fatty Acids / analysis
Fresh Water
Microcystis* / genetics
Nucleic Acid Hybridization
Phospholipids / analysis
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA

Substances

DNA, Bacterial
Fatty Acids
Phospholipids
RNA, Ribosomal, 16S