Imaging Fluorescent Nuclear Pore Complex Proteins in C. elegans

Methods Mol Biol. 2022:2502:373-393. doi: 10.1007/978-1-0716-2337-4_24.

Abstract

C. elegans is a well-characterized and relatively simple model organism, making it attractive for studying nuclear pore complex proteins in cell and developmental biology. C. elegans is transparent and highly amendable to genetic manipulation. Therefore, it is possible to generate fluorescently tagged proteins and combine this with various light microscopy techniques to study protein behavior in space and time. Here, we provide protocols to prepare both fixed and live C. elegans for confocal and light sheet microscopy. This enables the analysis of nuclear pore complex proteins from embryonic stages to the aging adult.

Keywords: 3D-SIM; C. elegans; Confocal; Fluorescence microscopy; Light sheet; Nuclear pore complex; Nucleus; Superresolution; fluorescent protein.

MeSH terms

  • Animals
  • Caenorhabditis elegans Proteins* / genetics
  • Caenorhabditis elegans Proteins* / metabolism
  • Caenorhabditis elegans* / genetics
  • Caenorhabditis elegans* / metabolism
  • Microscopy, Fluorescence / methods
  • Nuclear Pore Complex Proteins* / metabolism

Substances

  • Caenorhabditis elegans Proteins
  • Nuclear Pore Complex Proteins