Protocol to assess human glioma propagating cell migration on linear micropatterns mimicking brain invasion tracks

Michele Crestani; Tania Dini; Nils C Gauthier; Pascale Monzo

doi:10.1016/j.xpro.2022.101331

Protocol to assess human glioma propagating cell migration on linear micropatterns mimicking brain invasion tracks

STAR Protoc. 2022 Apr 18;3(2):101331. doi: 10.1016/j.xpro.2022.101331. eCollection 2022 Jun 17.

Authors

Michele Crestani¹, Tania Dini¹, Nils C Gauthier¹, Pascale Monzo¹

Affiliation

¹ IFOM - the Firc Institute of Molecular Oncology, Via Adamello, 16, 20139 Milan, Italy.

Abstract

Glioblastoma (GBM) cells invade the brain by following linear structures like blood vessel walls and white matter tracts by using specific motility modes. In this protocol, we describe two micropatterning techniques allowing recapitulation of these linear tracks in vitro: micro-contact printing and deep UV photolithography. We also detail how to maintain, transfect, and prepare human glioma propagating cells (hGPCs) for migration assays on linear tracks, followed by image acquisition and analysis, to measure key parameters of their motility. For complete details on the use and execution of this protocol, please refer to Monzo et al. (2016) and Monzo et al. (2021a).

Keywords: Biophysics; Biotechnology and bioengineering; Cancer; Cell Biology; Cell culture; Cell-based Assays; Microscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brain
Brain Neoplasms*
Cell Movement
Glioblastoma*
Glioma*
Humans