Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics

Methods Mol Biol. 2022:2456:53-62. doi: 10.1007/978-1-0716-2124-0_4.

Abstract

Mass spectrometry (MS) is a routinely used approach to characterize global protein profile in various biological samples. Here we describe rodent lung tissue homogenization, sample preparation, and liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for shotgun proteomics.

Keywords: Mass spectrometry; Rodent lungs; Sample preparation; Shotgun proteomics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Liquid / methods
  • Lung
  • Proteomics* / methods
  • Rodentia
  • Tandem Mass Spectrometry*

Grants and funding