iPSC-Derived Micro-Heart Muscle for Medium-Throughput Pharmacology and Pharmacogenomic Studies

Daniel W Simmons; Nathaniel Huebsch

doi:10.1007/978-1-0716-2261-2_8

iPSC-Derived Micro-Heart Muscle for Medium-Throughput Pharmacology and Pharmacogenomic Studies

Methods Mol Biol. 2022:2485:111-131. doi: 10.1007/978-1-0716-2261-2_8.

Authors

Daniel W Simmons^{1

2}, Nathaniel Huebsch^{3

4

5}

Affiliations

¹ Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
² NSF Science and Technology Center for Engineering Mechanobiology, Washington University in St. Louis, St. Louis, MO, USA.
³ Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA. nhuebsch@wustl.edu.
⁴ NSF Science and Technology Center for Engineering Mechanobiology, Washington University in St. Louis, St. Louis, MO, USA. nhuebsch@wustl.edu.
⁵ Center for Cardiovascular Research, Center for Regenerative Medicine, Center for Investigation of Membrane Excitability Diseases, Washington University in St. Louis, St. Louis, MO, USA. nhuebsch@wustl.edu.

PMID: 35618902
DOI: 10.1007/978-1-0716-2261-2_8

Abstract

Micro-heart muscle arrays enable medium-throughput experiments to model the cardiac response to a variety of environmental and pharmaceutical effects. Here, we describe stem cell culture maintenance, methods for successful cardiac differentiation, and formation of micro-heart muscle arrays for electrophysiology and molecular biology assays.

Keywords: Cardiac differentiation; Electrophysiology; Induced pluripotent stem cells (iPSC); Micro-heart muscle (μHM); Pharmacology.

MeSH terms

Cell Differentiation
Culture Media
Induced Pluripotent Stem Cells*
Myocardium
Pharmacogenomic Testing

Substances

Culture Media