Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite

In Kwon Choi; Xiaowen Liu

doi:10.1007/978-1-0716-2325-1_8

Top-Down Mass Spectrometry Data Analysis Using TopPIC Suite

Methods Mol Biol. 2022:2500:83-103. doi: 10.1007/978-1-0716-2325-1_8.

Authors

In Kwon Choi¹, Xiaowen Liu²

Affiliations

¹ Deming Department of Medicine, School of Medicine, Tulane University, New Orleans, LA, USA.
² Deming Department of Medicine, School of Medicine, Tulane University, New Orleans, LA, USA. xwliu@tulane.edu.

PMID: 35657589
DOI: 10.1007/978-1-0716-2325-1_8

Abstract

With the advances of mass spectrometry (MS) techniques, top-down MS-based proteomics has gained increasing attention because of its advantages over bottom-up MS in studying complex proteoforms. TopPIC Suite is a widely used software package for top-down MS-based proteoform identification and quantification. Here, we present the methods for top-down MS data analysis using TopPIC Suite.

Keywords: Proteoform identification; Proteoform quantification; Proteomics; Spectral deconvolution; Top-down mass spectrometry.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Data Analysis
Proteome* / analysis
Proteomics / methods
Software
Tandem Mass Spectrometry* / methods

Substances

Proteome

Abstract

Publication types

MeSH terms

Substances

Grants and funding