Normalization of maternal adiponectin in obese pregnant mice prevents programming of impaired glucose metabolism in adult offspring

FASEB J. 2022 Jul;36(7):e22383. doi: 10.1096/fj.202200326R.

Abstract

Infants born to obese mothers have a greater risk for childhood obesity and insulin resistance. However, the underlying biological mechanism remains elusive, which constitutes a significant roadblock for developing specific prevention strategies. Maternal adiponectin levels are lower in obese pregnant women, which is linked with increased placental nutrient transport and fetal overgrowth. We have previously reported that adiponectin supplementation to obese dams during the last four days of pregnancy prevented the development of obesity, glucose intolerance, muscle insulin resistance, and fatty liver in three months old offspring. In the present study, we tested the hypothesis that 6-9-month-old offspring of obese dams show glucose intolerance associated with muscle insulin resistance and mitochondrial dysfunction and that normalization of maternal adiponectin in obese pregnant mice prevents the development of this phenotype in the offspring. Male and female offspring of obese mice exhibited in vivo glucose intolerance and insulin resistance at 6 and 9 months of age. In gastrocnemius muscles ex vivo, male and female offspring of obese dams showed reduced phosphorylation of insulin receptor substrate 1Tyr-608 , AktThr-308 , and decreased Glut4 plasma membrane translocation upon insulin stimulation. These metabolic abnormalities in offspring born to obese mice were largely prevented by normalization of maternal adiponectin levels in late pregnancy. We provide evidence that low circulating maternal adiponectin is a critical mechanistic link between maternal obesity and the development of metabolic disease in offspring. Strategies aimed at improving maternal adiponectin levels may prevent long-term metabolic dysfunction in offspring of obese mothers.

Keywords: fetal programming; glucose intolerance; insulin resistance; metabolic diseases.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adiponectin / metabolism
  • Animals
  • Diabetes, Gestational* / metabolism
  • Female
  • Fetal Macrosomia / metabolism
  • Glucose / metabolism
  • Glucose Intolerance* / metabolism
  • Glucose Intolerance* / prevention & control
  • Insulin / metabolism
  • Insulin Resistance*
  • Male
  • Mice
  • Mice, Obese
  • Placenta / metabolism
  • Pregnancy

Substances

  • Adiponectin
  • Insulin
  • Glucose