MiR-743a-5p regulates differentiation of myoblast by targeting Mob1b in skeletal muscle development and regeneration

YongSheng Zhang; YiLong Yao; ZiShuai Wang; Dan Lu; YuanYuan Zhang; Adeyinka Abiola Adetula; SiYuan Liu; Min Zhu; YaLan Yang; XinHao Fan; MuYa Chen; YiJie Tang; Yun Chen; YuWen Liu; GuoQiang Yi; ZhongLin Tang

doi:10.1016/j.gendis.2020.11.018

MiR-743a-5p regulates differentiation of myoblast by targeting Mob1b in skeletal muscle development and regeneration

Genes Dis. 2020 Dec 3;9(4):1038-1048. doi: 10.1016/j.gendis.2020.11.018. eCollection 2022 Jul.

Authors

YongSheng Zhang^{1

2}, YiLong Yao^{1

2}, ZiShuai Wang^{1

2}, Dan Lu^{1

2}, YuanYuan Zhang³, Adeyinka Abiola Adetula^{1

2}, SiYuan Liu^{1

2}, Min Zhu^{1

2}, YaLan Yang^{1

2}, XinHao Fan^{1

2}, MuYa Chen^{1

2}, YiJie Tang^{1

2}, Yun Chen^{1

2}, YuWen Liu^{1

2}, GuoQiang Yi^{1

2}, ZhongLin Tang^{1

2

3}

Affiliations

¹ Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518124, PR China.
² Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518124, PR China.
³ Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.

Abstract

The microRNAs (miRNAs) play an important role in regulating myogenesis by targeting mRNA. However, the understanding of miRNAs in skeletal muscle development and diseases is unclear. In this study, we firstly performed the transcriptome profiling in differentiating C2C12 myoblast cells. Totally, we identified 187 miRNAs and 4260 mRNAs significantly differentially expressed that were involved in myoblast differentiation. We carried out validation of microarray data based on 5 mRNAs and 5 miRNAs differentially expressed and got a consistent result. Then we constructed and validated the significantly up- and down-regulated mRNA-miRNA interaction networks. Four interaction pairs (miR-145a-5p-Fscn1, miR-200c-5p-Tmigd1, miR-27a-5p-Sln and miR-743a-5p-Mob1b) with targeted relationships in differentiated myoblast cells were demonstrated. They are all closely related to myoblast development. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis indicated cell cycle signals important for exploring skeletal muscle development and disease. Functionally, we discovered that miR-743a targeting gene Mps One Binder Kinase Activator-Like 1B (Mob1b) gene in differentiated C2C12. The up-regulated miR-743a can promote the differentiation of C2C12 myoblast. While the down-regulated Mob1b plays a negative role in differentiation. In addition, the expression profile of miR-743a and Mob1b are consistent with skeletal muscle recovery after Cardiotoxin (CTX) injury. Our study revealed that miR-743a-5p regulates myoblast differentiation by targeting Mob1b involved in skeletal muscle development and regeneration. Our findings made a further exploration for mechanisms in myogenesis and might provide potential possible miRNA-based target therapies for skeletal muscle regeneration and disease in the near future.

Keywords: Differentiation; Mob1b; Myoblast; Skeletal muscle; Transcriptome; miR-743a-5p.