Adherence of Candida albicans and Malassezia Species to Skin Cells Induces Changes in the Expression of Genes Responsible for Heparan and Chondroitin Sulfate Chain Synthesis
Actas Dermosifiliogr. 2022 Jul-Aug;113(7):712-716.
doi: 10.1016/j.ad.2021.11.010.
Epub 2022 Mar 21.
[Article in
English,
Spanish]
Affiliations
- 1 Instituto Universitario Fernández-Vega (IUFV), Universidad de Oviedo, Oviedo, España; Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, España; Instituto de Investigación Sanitaria del Principado de Asturias, Oviedo, España.
- 2 Servicio de Dermatología, Hospital Universitario Central de Asturias, Oviedo, España; Departamento de Medicina, Universidad de Oviedo, Oviedo, España.
- 3 Instituto Universitario Fernández-Vega (IUFV), Universidad de Oviedo, Oviedo, España; Centro Comunitario de Sangre y Tejidos del Principado de Asturias, Oviedo, España.
- 4 Servicio de Dermatología, Hospital Universitario Central de Asturias, Oviedo, España.
- 5 Instituto Universitario Fernández-Vega (IUFV), Universidad de Oviedo, Oviedo, España; Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, España; Servicio de Microbiología, Hospital Universitario Central de Asturias, Oviedo, España.
- 6 Instituto Universitario Fernández-Vega (IUFV), Universidad de Oviedo, Oviedo, España; Departamento de Biología Funcional, Universidad de Oviedo, Oviedo, España; Instituto de Investigación Sanitaria del Principado de Asturias, Oviedo, España. Electronic address: cmartincueto@gmail.com.
Abstract
Superficial fungal infections are common in dermatology and are often caused by opportunistic species in the Candida and Malassezia genera. The aim of this study was to analyze changes in the expression of genes coding for enzymes involved in the biosynthesis of glycosaminoglycans (GAGs) chains following the adherence of Candida and Malassezia yeasts to skin cell lines. Gene expression was analyzed using reverse transcriptase-quantitative polymerase chain reaction assays. Interactions between the yeasts and the skin cells induced the following changes in genes involved in the biosynthesis of heparan sulfate and chondroitin sulfate: downregulation of CHPF in keratinocytes and downregulation of EXT1, EXT2, CHSY3, and CHPF in fibroblasts. Adherence to fibroblasts had an even greater effect on GAG biosynthetic enzymes, inducing the downregulation of 13 genes and the upregulation of two (CHST15 and CHST7). Interactions between yeasts and skin cells might affect the binding affinity of GAG chains, possibly changing their ability to function as receptors for pathogens and interfering with a key stage at the start of infection.
Keywords:
Chondroitin sulfates; Condroitín sulfato; Glucosaminoglucanos; Glycosaminoglycans; Heparan sulfate; Heparán sulfato; Infección; Infections; Reverse transcriptase polymerase chain reaction; qRT-PCR.
Copyright © 2022 AEDV. Publicado por Elsevier España, S.L.U. All rights reserved.
MeSH terms
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Candida albicans / genetics
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Candida albicans / metabolism
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Chondroitin Sulfates* / analysis
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Glycosaminoglycans / analysis
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Glycosaminoglycans / metabolism
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Heparitin Sulfate / analysis
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Heparitin Sulfate / metabolism
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Humans
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Malassezia* / genetics
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Malassezia* / metabolism
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Membrane Glycoproteins
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Sulfotransferases
Substances
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CHST15 protein, human
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Glycosaminoglycans
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Membrane Glycoproteins
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Chondroitin Sulfates
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Heparitin Sulfate
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Sulfotransferases