Profiling the Regulation of Histone Methylation and Demethylation by Metabolites and Metals

Sebastian Müller; Fabien Sindikubwabo; Tatiana Cañeque; Raphaël Rodriguez

doi:10.1007/978-1-0716-2481-4_6

Profiling the Regulation of Histone Methylation and Demethylation by Metabolites and Metals

Methods Mol Biol. 2022:2529:121-133. doi: 10.1007/978-1-0716-2481-4_6.

Authors

Sebastian Müller^{1

2

3}, Fabien Sindikubwabo^{1

2

3}, Tatiana Cañeque^{1

2

3}, Raphaël Rodriguez^{4

5

6}

Affiliations

¹ Institut Curie, 26 rue d'Ulm, Paris, France.
² PSL Université, Paris, France.
³ Chemical Biology of Cancer Laboratory, CNRS UMR 3666, INSERM U1143, Paris, France.
⁴ Institut Curie, Paris, France. Raphael.Rodriguez@curie.fr.
⁵ Paris Sciences et Lettres Research University, Sorbonne University, Paris, France. Raphael.Rodriguez@curie.fr.
⁶ INSERM U934/CNRS UMR 3215, Paris, France. Raphael.Rodriguez@curie.fr.

PMID: 35733013
DOI: 10.1007/978-1-0716-2481-4_6

Abstract

Here we describe how to profile the contribution of metabolism and implication of metals to histone methylation and demethylation. The techniques described with the adequate protocols are metabolomics, quantitative proteomics, inductively coupled mass spectrometry and nanoscale secondary ion mass spectrometry.

Keywords: Histone demethylation; Histone methylation; ICP-MS; Metabolomics; Metals; NanoSIMS; Proteomics.

MeSH terms

Demethylation
Histones* / metabolism
Metals* / metabolism
Methylation
Spectrometry, Mass, Secondary Ion

Substances

Histones
Metals