Platelet-rich Fibrin Promotes the Proliferation and Osteo-/odontoblastic Differentiation of Human Dental Pulp Stem Cells

Curr Stem Cell Res Ther. 2023;18(4):560-567. doi: 10.2174/1574888X17666220704092411.

Abstract

Background: Pulp regeneration is a promising strategy that promotes the continued development of young permanent teeth with immature apical foramen. Platelet-rich fibrin (PRF) was found to stimulate the proliferation and differentiation of osteoblasts, but its effects on osteoblast/odontoblast differentiation of human dental pulp stem cells (hDPSCs) are unknown.

Methods: The hDPSCs were isolated and identified using known surface markers by flow cytometry. The CCK-8 assay and the expression of Ki67 and PCNA were used to examine hDPSC proliferation. After 7 days of culture in an osteo-/odontoblastic induction medium with various concentrations of liquid PRF (0, 10% and 20%), the early stage of osteogenesis-intracellular alkaline phosphatase (ALP) was checked. After 21 days of culture, matrix mineralization was checked using Alizarin Red S and quantified. The mRNA and protein levels of osteo-/odontoblastic genes, including RUNX2, DSPP, DMP1 and BSP, were measured by qRT-PCR. The notch signal was checked by Western blot to analyze three key proteins (Notch 1, Jagged 1 and Hes 1).

Results: PRF-treated groups showed higher expression of Ki-67 and PCNA, higher ALP activity, and the higher dose showed a stronger induction. PRF promoted osteo-/odontoblastic differentiation of hDPSCs indicated by elevated protein levels and mRNA levels of the expression of osteo-/odontoblastic markers. The three key proteins in Notch signaling showed an increase compared with the control group and increased as the PRF concentration increased.

Conclusion: PRF can promote the proliferation and osteo-/odontoblastic differentiation of hDPSC, which may be through the Notch signaling pathway.

Keywords: Platelet-rich fibrin; apical periodontitis; immature apical foramen; notch signaling; osteogenesis; pulp regeneration.

MeSH terms

  • Cell Differentiation
  • Cell Proliferation
  • Cells, Cultured
  • Dental Pulp
  • Humans
  • Odontoblasts / metabolism
  • Platelet-Rich Fibrin*
  • Proliferating Cell Nuclear Antigen / metabolism
  • Proliferating Cell Nuclear Antigen / pharmacology
  • Regeneration
  • Stem Cells

Substances

  • Proliferating Cell Nuclear Antigen