Mitochondrial Ca2+ uptake by the MCU facilitates pyramidal neuron excitability and metabolism during action potential firing

Abstract

Neuronal activation is fundamental to information processing by the brain and requires mitochondrial energy metabolism. Mitochondrial Ca²⁺ uptake by the mitochondrial Ca²⁺ uniporter (MCU) has long been implicated in the control of energy metabolism and intracellular Ca²⁺ signalling, but its importance to neuronal function in the brain remains unclear. Here, we used in situ electrophysiology and two-photon imaging of mitochondrial Ca²⁺, cytosolic Ca²⁺, and NAD(P)H to test the relevance of MCU activation to pyramidal neuron Ca²⁺ signalling and energy metabolism during action potential firing. We demonstrate that mitochondrial Ca²⁺ uptake by the MCU is tuned to enhanced firing rate and the strength of this relationship varied between neurons of discrete brain regions. MCU activation promoted electron transport chain activity and chemical reduction of NAD⁺ to NADH. Moreover, Ca²⁺ buffering by mitochondria attenuated cytosolic Ca²⁺ signals and thereby reduced the coupling between activity and the slow afterhyperpolarization, a ubiquitous regulator of excitability. Collectively, we demonstrate that the MCU is engaged by accelerated spike frequency to facilitate neuronal activity through simultaneous control of energy metabolism and excitability. As such, the MCU is situated to promote brain functions associated with high frequency signalling and may represent a target for controlling excessive neuronal activity.