Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry

Jennifer Arcuri; Sanjoy K Bhattacharya

doi:10.1007/978-1-0716-2966-6_5

Characterization of Gangliosides from Mouse Optic Nerve Samples Using Mass Spectrometry

Methods Mol Biol. 2023:2625:65-69. doi: 10.1007/978-1-0716-2966-6_5.

Authors

Jennifer Arcuri^{1

2

3}, Sanjoy K Bhattacharya^{4

5

6}

Affiliations

¹ Bascom Palmer Eye Institute & Department of Ophthalmology, University of Miami, Miami, FL, USA.
² Molecular and Cellular Pharmacology Graduate Program, University of Miami, Miami, FL, USA.
³ Miami Integrative Metabolomics Research Center, University of Miami, Miami, FL, USA.
⁴ Bascom Palmer Eye Institute & Department of Ophthalmology, University of Miami, Miami, FL, USA. sbhattacharya@med.miami.edu.
⁵ Molecular and Cellular Pharmacology Graduate Program, University of Miami, Miami, FL, USA. sbhattacharya@med.miami.edu.
⁶ Miami Integrative Metabolomics Research Center, University of Miami, Miami, FL, USA. sbhattacharya@med.miami.edu.

PMID: 36653632
DOI: 10.1007/978-1-0716-2966-6_5

Abstract

This chapter focuses on identifying gangliosides in the optic nerve of the mouse using mass spectrometry techniques. The described protocol will also permit the characterization of the sample's lipidome. Two deuterium-labeled ganglioside standards and a general lipid class standard will be utilized for extraction efficiency and quantification. Using reversed-phase high-performance liquid chromatography (HPLC) coupled to a Q Exactive mass spectrometer, the samples will be analyzed. The method will consist of both an untargeted approach and a targeted approach with a ganglioside-specific inclusion list.

Keywords: Gangliosides; Lipidomics; Mass spectrometry; Optic nerve.

MeSH terms

Animals
Chromatography, High Pressure Liquid / methods
Chromatography, Reverse-Phase* / methods
Gangliosides* / chemistry
Mass Spectrometry / methods
Mice
Optic Nerve / chemistry

Substances

Gangliosides