Vitamin D and its metabolites in the pollen of pine. Part 5: Steroid hormones in the pollen of pine species

M Saden-Krehula; M Tajíć

Vitamin D and its metabolites in the pollen of pine. Part 5: Steroid hormones in the pollen of pine species

Pharmazie. 1987 Jul;42(7):471-2.

Authors

M Saden-Krehula¹, M Tajíć

Affiliation

¹ Department of Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Yugoslavia.

PMID: 3671475

Abstract

Unconjugated vitamin D and its metabolites were investigated in the pollen of Pinus nigra Ar. and Pinus sylvestris L. by TLC, HPLC and competitive radiochemical determination of 25-hydroxycholecalciferol (25-OHD3). It was found that vitamin D (D2, D3) was present in the pollen in amounts about 2 micrograms/10 g and 25-OHD3, 24,25-dihydroxycholecalciferol [24,25-(OH)2D3] and 1,25-dihydroxycholecalciferol [1,25-(OH)2D3] between 0.1 and 3 micrograms/10 g of pollen, dependent on pollen species and methods.

MeSH terms

Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Plants / analysis*
Pollen / analysis*
Protein Binding
Steroids / analysis*
Vitamin D / analysis*

Substances

Steroids
Vitamin D