Long-Read Single-Cell Sequencing Using scCOLOR-seq

Martin Philpott; Udo Oppermann; Adam P Cribbs

doi:10.1007/978-1-0716-2996-3_18

Long-Read Single-Cell Sequencing Using scCOLOR-seq

Methods Mol Biol. 2023:2632:259-267. doi: 10.1007/978-1-0716-2996-3_18.

Authors

Martin Philpott¹, Udo Oppermann^{1

2}, Adam P Cribbs^{3

4}

Affiliations

¹ Botnar Research Centre, Nuffield Department of Orthopedics, Rheumatology and Musculoskeletal Sciences, National Institute of Health Research Oxford Biomedical Research Unit (BRU), University of Oxford, Oxford, UK.
² Oxford Centre for Translational Myeloma Research University of Oxford, Oxford, UK.
³ Botnar Research Centre, Nuffield Department of Orthopedics, Rheumatology and Musculoskeletal Sciences, National Institute of Health Research Oxford Biomedical Research Unit (BRU), University of Oxford, Oxford, UK. adam.cribbs@ndorms.ox.ac.uk.
⁴ Oxford Centre for Translational Myeloma Research University of Oxford, Oxford, UK. adam.cribbs@ndorms.ox.ac.uk.

PMID: 36781734
DOI: 10.1007/978-1-0716-2996-3_18

Abstract

Single-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its application to third-generation sequencing platforms such as Oxford Nanopore has been challenging because of its lower basecalling accuracy. Here we describe the method to perform highly accurate single-cell COrrected Long-Read sequencing (scCOLOR-seq) by droplet-based encapsulation of cells and sequencing using the Oxford Nanopore Sequencing system.

Keywords: Long-read; Oxford Nanopore Sequencing; Single-cell.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

High-Throughput Nucleotide Sequencing* / methods
Nanopores*
Sequence Analysis, DNA / methods

Abstract

Publication types

MeSH terms

Grants and funding