Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer

Meilian Zhuo; Jiangjiao Mao; Charles J David

doi:10.1016/j.xpro.2023.102108

Protocol to package and concentrate adeno-associated virus serotype 8 for use in autochthonous mouse models of pancreatic cancer

STAR Protoc. 2023 Mar 17;4(1):102108. doi: 10.1016/j.xpro.2023.102108. Epub 2023 Feb 10.

Authors

Meilian Zhuo¹, Jiangjiao Mao², Charles J David³

Affiliations

¹ Tsinghua University School of Medicine, Beijing, China; Peking University-Tsinghua Center for Life Sciences, Beijing, China. Electronic address: zhuoml21@163.com.
² Tsinghua University School of Medicine, Beijing, China.
³ Tsinghua University School of Medicine, Beijing, China; Peking University-Tsinghua Center for Life Sciences, Beijing, China. Electronic address: cdavid@mail.tsinghua.edu.cn.

Abstract

We have developed an economical and rapid protocol to package and concentrate adeno-associated virus serotype 8, allowing production of high-titer virus for use in vivo within 1 week. When combined with the CRISPR-Cas9 system, this provides a straightforward method for knockout of genes of interest in the pancreas. The method can also be used to express cDNAs in the pancreas. This method shows great potential to accelerate pancreatic cancer research in autochthonous models. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).¹.

Keywords: CRISPR; Cancer; Cell Biology; Cell culture; Cell-based Assays; Flow Cytometry/Mass Cytometry; Gene Expression; Genetics; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dependovirus* / genetics
Disease Models, Animal
Mice
Pancreas
Pancreatic Neoplasms* / genetics
Serogroup