Comparison of Intravenous Microdialysis and Standard Plasma Sampling for Monitoring of Vancomycin and Meropenem Plasma Concentrations-An Experimental Porcine Study

Johanne Gade Lilleøre; Sofus Vittrup; Sara Kousgaard Tøstesen; Pelle Hanberg; Maiken Stilling; Mats Bue

doi:10.3390/antibiotics12040791

Comparison of Intravenous Microdialysis and Standard Plasma Sampling for Monitoring of Vancomycin and Meropenem Plasma Concentrations-An Experimental Porcine Study

Antibiotics (Basel). 2023 Apr 21;12(4):791. doi: 10.3390/antibiotics12040791.

Authors

Johanne Gade Lilleøre^{1

2}, Sofus Vittrup^{1

2}, Sara Kousgaard Tøstesen^{1

2}, Pelle Hanberg^{1

2}, Maiken Stilling^{1

2

3}, Mats Bue^{1

2

3}

Affiliations

¹ Department of Clinical Medicine, Aarhus University, 8200 Aarhus, Denmark.
² Aarhus Denmark Microdialysis Research Group (ADMIRE), Aarhus University Hospital, 8200 Aarhus, Denmark.
³ Department of Orthopedic Surgery, Aarhus University Hospital, 8200 Aarhus, Denmark.

Abstract

Microdialysis is a catheter-based method suitable for dynamic sampling of unbound antibiotic concentrations. Intravenous antibiotic concentration sampling by microdialysis has several advantages and may be a superior alternative to standard plasma sampling. We aimed to compare concentrations obtained by continuous intravenous microdialysis sampling and by standard plasma sampling of both vancomycin and meropenem in a porcine model. Eight female pigs received 1 g of both vancomycin and meropenem, simultaneously over 100 and 10 min, respectively. Prior to drug infusion, an intravenous microdialysis catheter was placed in the subclavian vein. Microdialysates were collected for 8 h. From a central venous catheter, plasma samples were collected in the middle of every dialysate sampling interval. A higher area under the concentration/time curve and peak drug concentration were found in standard plasma samples compared to intravenous microdialysis samples, for both vancomycin and meropenem. Both vancomycin and meropenem concentrations obtained with intravenous microdialysis were generally lower than from standard plasma sampling. The differences in key pharmacokinetic parameters between the two sampling techniques underline the importance of further investigations to find the most suitable and reliable method for continuous intravenous antibiotic concentration sampling.

Keywords: meropenem; microdialysis; pharmacokinetics; plasma sampling; vancomycin.

Grants and funding

This research was funded by the Orthopedic Research Unit, Aarhus University Hospital. The funding party had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.