Measuring Immune Cell Movement Toward the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay

Eimear Mylod; Joanne Lysaght; Melissa J Conroy

doi:10.1007/978-1-0716-3056-3_14

Measuring Immune Cell Movement Toward the Soluble Microenvironment of Human Tissues Using a Boyden Chamber-Based Migration Assay

Methods Mol Biol. 2023:2645:231-240. doi: 10.1007/978-1-0716-3056-3_14.

Authors

Eimear Mylod¹, Joanne Lysaght¹, Melissa J Conroy²

Affiliations

¹ Cancer Immunology and Immunotherapy Group, Department of Surgery, Trinity Translational Medicine Institute, St James's Hospital, Dublin, Ireland.
² Cancer Immunology Research Group, Department of Physiology, School of Medicine, Trinity College Dublin, Dublin, Ireland. meconroy@tcd.ie.

PMID: 37202623
DOI: 10.1007/978-1-0716-3056-3_14

Abstract

Migration assays are used to measure cell movement toward a variety of chemoattractants in a controlled environment. Here we describe a method for a Boyden chamber-based migration assay using conditioned media generated from the tumor, liver, and visceral adipose tissue of cancer patients.

Keywords: Boyden chamber; Cell migration; Chemokines; Chemotaxis; Immune cells.

MeSH terms

Cell Line, Tumor
Cell Migration Assays / methods
Cell Movement
Chemotactic Factors*
Chemotaxis*
Culture Media, Conditioned
Humans

Substances

Chemotactic Factors
Culture Media, Conditioned