Lactiplantibacillus carotarum AMBF275T sp. nov. isolated from carrot juice fermentation

Abstract

A novel strain of the genus Lactiplantibacillus, named AMBF275^T, was isolated from fermented carrot juice, a salted fermented beverage dominated by lactic acid bacteria. The results of phylogenetic analysis indicated that the 16S rRNA gene of AMBF275^T is most similar to the 16S rRNA gene of Lactiplantibacillus garii FI11369^T with a sequence similarity of 99.4 %. However, a genome-wide comparison using average nucleotide identity (ANI) revealed that AMBF275^T and L. garii FI11369^T have an ANI of only 82.35 %. ANI values between AMBF275^T and other representative strains of species of the genus Lactiplantibacillus from the Genome Taxonomy Database (GTDB) were even lower than this 82.35 %, indicating that AMBF275^T represents a distinct species. We thus propose the name Lactiplantibacillus carotarum sp. nov. for this novel species, with AMBF275^T (=LMG 32885^T, =CECT 30757^T) as the type strain.

Keywords: Citizen-Science; Genome-Wide Comparison; Lactiplantibacillus; novel species.