Monitoring Lysosome Function in Ferroptosis

Fangquan Chen; Rui Kang; Daolin Tang; Jiao Liu

doi:10.1007/978-1-0716-3433-2_9

Monitoring Lysosome Function in Ferroptosis

Methods Mol Biol. 2023:2712:91-102. doi: 10.1007/978-1-0716-3433-2_9.

Authors

Fangquan Chen¹, Rui Kang², Daolin Tang², Jiao Liu³

Affiliations

¹ DAMP Laboratory, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.
² Department of Surgery, UT Southwestern Medical Center, Dallas, TX, USA.
³ DAMP Laboratory, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China. 2018683073@gzhmu.edu.cn.

PMID: 37578699
DOI: 10.1007/978-1-0716-3433-2_9

Abstract

Ferroptosis is a type of regulated cell death that occurs due to iron-induced membrane lipid peroxidation. Lysosomes, which are acidic, membrane-bound organelles containing various hydrolases, play a vital role in ferroptosis. They not only aid in the degradation of autophagic substrates, but also serve as signaling hubs in cell death. Specifically, lysosomes are involved in the induction and execution of ferroptosis through autophagy-mediated degradation of anti-ferroptotic proteins, lysosomal membrane permeability-mediated release of cathepsins, and iron-induced lysosomal membrane lipid peroxidation. Therefore, it is essential to have reliable methods for monitoring lysosomal functions, including lysosomal activity, pH, and membrane integrity, as well as iron accumulation and lipid peroxidation, to understand ferroptosis. This chapter introduces several protocols, such as western blotting, immunofluorescence, lysosomal probes, and lipid peroxidation assay kits, for monitoring the process of lysosome-related ferroptosis.

Keywords: Autophagy; Cell death; Ferroptosis; Lysosome.