Effects of different diets on Aedes aegypti adults: improving rearing techniques for sterile insect technique

Bull Entomol Res. 2023 Dec;113(6):748-755. doi: 10.1017/S0007485323000408. Epub 2023 Sep 25.

Abstract

The aim was to evaluate the effect of different energy diets available in adulthood on the longevity, dispersal capacity and sexual performance of Aedes aegypti produced under a mass-rearing system. To evaluate the effects of diets in relation to the survival of the adult male insects of Ae. aegypti, six treatments were used: sucrose at a concentration of 10%, as a positive control (sack10); starvation, as a negative control (starvation); sucrose at a concentration of 20% associated with 1 g/l of ascorbic acid (sac20vitC); wild honey in a concentration of 10% (honey10); demerara sugar in a 10% concentration (demerara10); and sucrose at a concentration of 20% associated with 1 g/l of ascorbic acid and 0.5 g/l of amino acid proline (sac20vitCPr). Each treatment had 16 cages containing 50 adult males. For the tests of flight ability and propensity to copulation, five treatments were used (saca10; sac20vitC; mel10; demerara10; and sac20vitCPr), with males each for flight ability and females copulated by a single male for copulation propensity. The diet composed of sucrose at a concentration of 20% associated with ascorbic acid, as an antioxidant, improved the survival, flight ability and propensity to copulate in Ae. aegypti males under mass-rearing conditions, and may be useful to enhance the performance of sterile males, thus improving the success of sterile insect technique programmes.

Keywords: copulation propensity; flight ability; sterile male; survival.

MeSH terms

  • Aedes*
  • Animals
  • Ascorbic Acid
  • Diet
  • Female
  • Insecta
  • Male
  • Mosquito Control / methods
  • Sucrose

Substances

  • Ascorbic Acid
  • Sucrose