Generation and ex vivo culture of murine and human pancreatic ductal adenocarcinoma tissue slice explants

Abstract

Traditional 2D/3D co-culture models typically do not reflect the cellular heterogeneity of pancreatic ductal adenocarcinoma (PDAC) tumors, while in vivo models can be slow and ill-suited to mechanistic investigations. Here, we present a protocol for culturing murine PDAC explants and a corresponding human PDAC model using tissue slice explants. We describe steps for sponge production, preparation of media and materials, tissue collection, and sectioning. We then detail procedures for explant plating, daily culture, and collection of samples.

Keywords: Biotechnology and bioenginee; Cancer; Cell culture; Model Organisms; Tissue Engineering.