Upregulation of pmrA, pmrB, pmrC, phoQ, phoP, and arnT genes contributing to resistance to colistin in superbug Klebsiella pneumoniae isolates from human clinical samples in Tehran, Iran

Maryam Mirshekar; Rezvan Golmoradi Zadeh; Majid Taati Moghadam; Shahla Shahbazi; Faramarz Masjedian Jazi

doi:10.1016/j.nmni.2024.101275

Upregulation of pmrA, pmrB, pmrC, phoQ, phoP, and arnT genes contributing to resistance to colistin in superbug Klebsiella pneumoniae isolates from human clinical samples in Tehran, Iran

New Microbes New Infect. 2024 Apr 1:59:101275. doi: 10.1016/j.nmni.2024.101275. eCollection 2024 Jun.

Authors

Maryam Mirshekar¹, Rezvan Golmoradi Zadeh¹, Majid Taati Moghadam², Shahla Shahbazi³, Faramarz Masjedian Jazi^{1

4}

Affiliations

¹ Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
² Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
³ Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave., Tehran, 13164, Iran.
⁴ Student Research Committee, Iran University of Medical Sciences, Tehran, Iran.

Abstract

Background: Antibiotic resistance in Klebsiella pneumoniae isolates, particularly resistance to colistin, has become a growing concern. This study seeks to investigate the upregulation of specific genes (pmrA, pmrB, pmrC, phoQ, phoP, and arnT) that contribute to colistin resistance in K. pneumoniae isolates collected from human clinical samples in Tehran, Iran.

Methods: Thirty eight K. pneumoniae isolates were obtained and subjected to antibiotic susceptibility testing, as well as evaluation for phenotypic AmpC and ESBL production according to CLSI guidelines. The investigation of antibiotic resistance genes was conducted using polymerase chain reaction (PCR), whereas the quantification of colistin resistance related genes expressions was performed via Real-Time PCR.

Results: The highest and lowest antibiotics resistance were observed for cefotaxime 33 (86.8%) and minocycline 8 (21.1%), respectively. Twenty-four (63.2%) and 31 (81.6%) isolates carried AmpC and ESBLs, respectively. Also, antibiotic resistance genes containing bla_NDM, bla_IMP, bla_VIM, bla_SHV, bla_TEM, bla_CTXM, qnrA, qnrB, qnrS, and aac(6')-Ib were detected in K. pneumoniae isolates. Only 5 (13.1%) isolates were resistant to colistin and the MIC range of these isolates was between 4 and 64 μg ml^-1. Upregulation of the pmrA, pmrB, pmrC, phoQ, phoP, and arnT genes was observed in colistin-resistant isolates. The colistin-resistant isolates were found to possess a simultaneous presence of ESBLs, AmpC, fluoroquinolone, aminoglycoside, and carbapenem resistant genes.

Conclusions: This study reveals escalating antibiotic resistance in K. pneumoniae, with notable coexistence of various resistance traits, emphasizing the need for vigilant surveillance and innovative interventions.

Keywords: AmpC; Carbapenemases; Colistin-resistant; ESBL; Klebsiella pneumoniae.