In Experiment I normal subjects performed the classical clinical line-bisection task, and demonstrated a left-side underestimation. In Experiment II subjects maintained fixation upon a central position and adjusted a rod passing through this fixation point so that both extremities were judged equal. The left-side underestimation was very much greater under these conditions, but was considerably reduced when retinal and gravitational coordinates were dissociated by making the subjects lie horizontally on one or other side. Subjects then demonstrated greater left-side and top-half underestimation when lying on the left than on the right side. Gravitational coordinates and the apparent locus of events in extrapersonal space are determinants of perceptual asymmetries at least as important as anatomical connectivities.