Abstract
Cell-free extracts prepared from a Pseudomonas sp., grown on (+)-catechin, oxidized dihydrogossypetin (3',4',5,7,8-pentahydroxyflavanonol) by cleaving the A-ring to form oxaloacetic acid from C-5, C-6, C-7 and C-8 together with 5-(3,4-dihydroxyphenyl)-4-hydroxy-3-oxovalero-delta-lactone. The structure of this lactone was confirmed by synthesis of related phenylvalerolactones.
MeSH terms
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Carbon Isotopes
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Cell-Free System
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Chromatography, Thin Layer
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Edetic Acid
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Flavonoids / metabolism*
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Lactones / biosynthesis
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Lactones / chemical synthesis
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Mass Spectrometry
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Oxaloacetates / biosynthesis
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Oxidation-Reduction
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Pseudomonas / metabolism*
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Pyruvates / analysis
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Soil Microbiology
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Spectrophotometry
Substances
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Carbon Isotopes
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Flavonoids
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Lactones
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Oxaloacetates
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Pyruvates
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Edetic Acid