Dissociation and interaction of individual components of a degradative plasmid aggregate in Pseudomonas

Proc Natl Acad Sci U S A. 1974 Sep;71(9):3410-4. doi: 10.1073/pnas.71.9.3410.

Abstract

The transfer of the OCT plasmid from Pseudomonas oleovorans to Pseudomonas putida strain PpGl results in the acquisition of three independent replicons: OCT, factor K, and the MER plasmid. OCT is a nontransmissible plasmid harboring genes that code for the enzymes responsible for the degradation of n-octane. Factor K is a transfer plasmid capable of mobilizing OCT as well as chromosomal genes but incapable of enhancing transfer frequencies of other transmissible plasmids such as CAM, SAL, or RP-1. MER is a self-transmissible plasmid which can confer resistance to high concentrations of mercury salts. While OCT and MER are incompatible with CAM, factor K is compatible with it. Transmissible plasmids such as SAL, CAM, MER, or RP-1 cannot mobilize OCT to any significant extent, and exert strong repression on factor K-mediated transfer of chromosomal genes.

MeSH terms

  • Alkanes / metabolism
  • Biotransformation
  • Conjugation, Genetic
  • Drug Resistance, Microbial*
  • Extrachromosomal Inheritance*
  • Genes
  • Mercury / pharmacology*
  • Phenotype
  • Pseudomonas / metabolism*
  • Transduction, Genetic

Substances

  • Alkanes
  • Mercury