Abstract
The nucleotides ppGpp and pppGpp accumulate when wild-type, but not rel(-), strains of Escherichia coli are starved for required amino acids. These compounds are synthesized on ribosomes, in the presence of the product of the rel gene, from GDP and GTP; ATP is used as the phosphate donor. The signal for making these compounds is the presence of an uncharged tRNA in the ribosomal acceptor site. These compounds are not accumulated if the ribosomes are actively engaged in protein synthesis.
MeSH terms
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Adenosine Triphosphate
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Amino Acid Sequence
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Binding Sites
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Buffers
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Centrifugation, Density Gradient
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Chromatography, Gel
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Dialysis
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Escherichia coli / metabolism
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Guanine Nucleotides / biosynthesis*
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Guanosine Triphosphate
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Hydrolysis
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Peptide Initiation Factors / pharmacology
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Polynucleotides
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RNA, Bacterial / metabolism*
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RNA, Messenger / metabolism
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RNA, Transfer / metabolism*
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Ribosomes / metabolism*
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Rifampin / pharmacology
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Templates, Genetic
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Tetracycline / pharmacology
Substances
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Buffers
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Guanine Nucleotides
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Peptide Initiation Factors
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Polynucleotides
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RNA, Bacterial
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RNA, Messenger
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Guanosine Triphosphate
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Adenosine Triphosphate
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RNA, Transfer
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Tetracycline
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Rifampin