Abstract
The alpha-complementation reaction was adapted as a quantitative assay for fusion proteins consisting of the B chain of human insulin fused to the C terminus of the truncated beta-galactosidase.
MeSH terms
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Chromatography, High Pressure Liquid
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Cloning, Molecular*
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DNA Restriction Enzymes
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Galactosidases / genetics*
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Genes*
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Genes, Bacterial*
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Genetic Complementation Test
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Humans
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Insulin / genetics*
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Macromolecular Substances
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Plasmids
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beta-Galactosidase / genetics*
Substances
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Insulin
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Macromolecular Substances
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DNA Restriction Enzymes
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Galactosidases
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beta-Galactosidase