Abstract
Techniques for high frequency yeast transformation have been described. A double-strand break introduced by restriction enzyme cleavage can be used to direct a plasmid to integrate into a particular chromosomal locus. Plasmids containing a double-strand gap can be used in a straightforward method for the isolation and mapping of chromosomal alleles. These techniques extend the genetic applications of yeast transformation.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Alleles
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Chromosomes / physiology
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DNA Restriction Enzymes
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DNA, Bacterial / genetics*
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DNA, Fungal / genetics*
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Escherichia coli / genetics*
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Genetic Engineering / methods
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Genetic Vectors*
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Nucleic Acid Hybridization
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Plasmids*
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Saccharomyces cerevisiae / genetics*
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Transformation, Genetic*
Substances
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DNA, Bacterial
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DNA, Fungal
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DNA Restriction Enzymes