When a cloned 6 kb Eco RI-Sal I fragement of mouse ribosomal gene nontranscribed spacer DNA (rDNA NTS) was used to screen a BALB/c mouse gene library, 25% of the recombinant phage hybridized with it. In situ hybridization experiments and characterization of 12 clones selected using this probe supported the idea that sequences homologous to this rDNA NTS region are scattered throughout the genome. Subsequently, sequences homologous to mouse rDNA NTS were found flanking mouse mu, alpha and gamma 2b immunoglobulin CH genes. One region was localized 3' to the mu coding sequence, an area which has been identified as an intervening sequence between the secreted C mu heavy chain terminus and the C terminal portion of the membrane-bound C mu heavy chain.