Abstract
The purification and properties of a cytotoxic mouse monoclonal anti-HLA-A2 antibody are described. This antibody, BB7.2, can be used as an HLA-A2 typing reagent with little modification of current typing techniques. It also recognizes a low frequency variant of HLA-A28. BB7.2 provides an example of an antibody which requires bivalent attachment to a polymeric antigen, e.g., a cell, to produce a readily detectable complex. This is due to a high rate of dissociation of the complex formed between a single BB7.2 combining sites and HLA-A2. The consequences of this property were investigated and some complications for potential uses of such monoclonal antibodies are discussed.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / genetics
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Antibodies, Monoclonal / immunology
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Antibodies, Monoclonal / isolation & purification*
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Antibody Specificity*
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Antibody-Producing Cells / immunology
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Binding Sites, Antibody
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Binding, Competitive
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Carcinoma, Ehrlich Tumor / immunology
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Chemical Fractionation
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Cytotoxicity, Immunologic*
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Electrophoresis, Polyacrylamide Gel
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Genetic Variation
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HLA Antigens / genetics
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HLA Antigens / immunology*
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Humans
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Immunoglobulin Fab Fragments
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Immunoglobulin G / isolation & purification
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Kinetics
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Mice
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Mice, Inbred BALB C
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Peptide Hydrolases / pharmacology
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Rabbits
Substances
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Antibodies, Monoclonal
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HLA Antigens
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Immunoglobulin Fab Fragments
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Immunoglobulin G
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Peptide Hydrolases