The lysosomal proteinase of Artemia. Purification and characterization

Eur J Biochem. 1982 May 17;124(2):357-62. doi: 10.1111/j.1432-1033.1982.tb06600.x.

Abstract

One single lysosomal proteinase has been found in the dormant gastrulae of Artemia. The enzyme has been purified to homogeneity and characterized. It is a thiol, cathepsin-B-like proteinase with an apparent molecular weight of 68 000 and is composed of a single polypeptide chain. The proteinase is a glycoprotein, a characteristic feature of lysosomal enzymes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Artemia / enzymology
  • Cathepsin B
  • Cathepsins / isolation & purification
  • Cathepsins / metabolism
  • Glycoproteins / isolation & purification
  • Kinetics
  • Lysosomes / enzymology*
  • Molecular Weight
  • Peptide Hydrolases / isolation & purification*
  • Peptide Hydrolases / metabolism

Substances

  • Glycoproteins
  • Cathepsins
  • Peptide Hydrolases
  • Cathepsin B