Molecular cloning of a novel protein regulated by opioid treatment of NG108-15 cells

Brain Res Mol Brain Res. 1995 Aug;32(1):171-5. doi: 10.1016/0169-328x(95)00090-f.

Abstract

A new cDNA clone, NGD5, has been identified from a subtraction cDNA library constructed with mRNA isolated from control neuroblastoma x glioma NG108-15 cells and cells treated for 48 h with the delta-opioid agonist, D-Ala2, D-Leu5 enkephalin (DADLE). NGD5 mRNA is decreased, in a naloxone-reversible manner, upon long-term treatment of NG108-15 cells with DADLE, indicating that this clone may be related to opioid receptor function. Northern analysis indicates that NGD5 mRNA is expressed in rat brain. Two similar nearly full-length NGD5 clones, NGD5A and NGD5B, were isolated from a lambda gt10 NG108-15 cDNA library and sequenced. The predicted 40-kDa peptide encoded for by the NGD5 cDNA has no significant homology to the recently cloned mu, delta or kappa opioid receptors nor to any other known proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Down-Regulation / drug effects
  • Enkephalin, Leucine-2-Alanine / pharmacology*
  • Gene Expression Regulation, Neoplastic / physiology*
  • Hybrid Cells
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics*
  • Nerve Tissue Proteins / genetics*
  • Rats
  • Receptors, Opioid, delta / drug effects*
  • Tumor Cells, Cultured

Substances

  • Neoplasm Proteins
  • Nerve Tissue Proteins
  • Receptors, Opioid, delta
  • Enkephalin, Leucine-2-Alanine

Associated data

  • GENBANK/L38481