A serotype-specific epitope of dengue virus 1 identified by phage displayed random peptide library

FEMS Microbiol Lett. 1995 Mar 15;127(1-2):93-8. doi: 10.1111/j.1574-6968.1995.tb07455.x.

Abstract

From a panel of monoclonal antibodies of dengue viruses, a serotype-specific epitope of dengue virus 1 was screened from a random peptide library displayed on phage. The epitope was the determinant reactive with monoclonal antibody 15F3-1 that was specific to dengue 1. The screening was monitored by a dot blotting procedure, and after three rounds of screening a consensus motif, HRYSWK, was found. This sequence matches the sequence HKYSWK, corresponding to the amino acid residues 885-890 of polyprotein or residues 111-116 of the non-structural protein 1 of dengue virus serotype 1. The linear epitope was confirmed by testing the antigenicity of chemically synthesized 8-branched peptide.

MeSH terms

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Antigens, Viral / genetics*
  • Base Sequence
  • Binding, Competitive
  • Cloning, Molecular
  • Consensus Sequence
  • DNA Primers / genetics
  • DNA, Viral / genetics
  • Dengue Virus / classification
  • Dengue Virus / genetics*
  • Dengue Virus / immunology*
  • Epitopes / genetics
  • Gene Library
  • Molecular Sequence Data
  • Oligopeptides / genetics
  • Oligopeptides / immunology
  • Oligopeptides / metabolism
  • Serotyping
  • Viral Proteins / genetics
  • Viral Proteins / immunology
  • Viral Proteins / metabolism

Substances

  • Antibodies, Monoclonal
  • Antigens, Viral
  • DNA Primers
  • DNA, Viral
  • Epitopes
  • Oligopeptides
  • Viral Proteins