Abstract
The effects of high (28mM) D-glucose (HG) on the cell cycle progression were investigated in rat aorta vascular smooth muscle cells (VSMCs) in primary culture, using an immunocytochemical analysis of cell-cycle-specific nuclear antigens. HG had no effect on the cell cycle of the serum-deprived G0 cells, whereas platelet-derived growth factor (PDGF) stimulated the entry of the G0 cells to the G1 phase without a further progression to the S and M phases. HG, but neither mannitol nor L-glucose, stimulated the progression of the PDGF-pretreated G1 cells to the S and M phases, which was blocked by calphostin-C, a protein kinase C (PKC) blocker. HG did not affect the cytosolic Ca2+ concentration ([Ca2+]i). These data suggest that HG has no competent effect on the G0 cells and acts as a progression growth factor (to stimulate the cell cycle from the G1 to the S/M) in VSMCs through the mechanism, which may be insensitive to [Ca2+]i and mediated by PKC.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aorta / cytology
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Aorta / drug effects
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Becaplermin
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Cell Cycle / drug effects*
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Cells, Cultured
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Dose-Response Relationship, Drug
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Fluorescent Antibody Technique
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G1 Phase
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Glucose / pharmacology*
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Humans
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Ki-67 Antigen
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Kinetics
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Male
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Mannitol / pharmacology
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Mitosis
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Muscle, Smooth, Vascular / cytology*
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Muscle, Smooth, Vascular / drug effects
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Naphthalenes*
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Neoplasm Proteins / analysis
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Nickel / pharmacology
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Nuclear Proteins / analysis
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Platelet-Derived Growth Factor / pharmacology
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Polycyclic Compounds / pharmacology
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Proliferating Cell Nuclear Antigen / analysis
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Protein Kinase C / antagonists & inhibitors
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Proto-Oncogene Proteins c-sis
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Rats
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Rats, Wistar
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Recombinant Proteins / pharmacology
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Resting Phase, Cell Cycle
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S Phase
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Stereoisomerism
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Time Factors
Substances
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Ki-67 Antigen
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Naphthalenes
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Neoplasm Proteins
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Nuclear Proteins
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Platelet-Derived Growth Factor
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Polycyclic Compounds
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Proliferating Cell Nuclear Antigen
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Proto-Oncogene Proteins c-sis
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Recombinant Proteins
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Becaplermin
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Mannitol
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nickel chloride
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Nickel
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Protein Kinase C
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calphostin C
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Glucose