Abstract
The transcription of the human tenascin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significantly reduce this activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Animals
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Base Sequence
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Cell Adhesion Molecules, Neuronal / genetics*
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Chloramphenicol O-Acetyltransferase / genetics
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Cricetinae
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Enhancer Elements, Genetic / genetics*
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Exons / genetics*
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Extracellular Matrix Proteins / genetics*
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Genes / genetics
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Genes, Reporter / genetics
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Humans
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Mice
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Molecular Sequence Data
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Promoter Regions, Genetic / genetics
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RNA, Messenger / analysis
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Sequence Homology, Nucleic Acid
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Tenascin
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Transcription, Genetic / genetics*
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Transfection
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Tumor Cells, Cultured
Substances
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Cell Adhesion Molecules, Neuronal
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Extracellular Matrix Proteins
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RNA, Messenger
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Tenascin
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Chloramphenicol O-Acetyltransferase