The first untranslated exon of the human tenascin-C gene plays a regulatory role in gene transcription

FEBS Lett. 1995 Aug 7;369(2-3):335-9. doi: 10.1016/0014-5793(95)00778-8.

Abstract

The transcription of the human tenascin-C (TN-C) gene is directed by a single promoter. Here we demonstrate, in transiently transfected cells, that two distinct regions of the untranslated 179 bp-long exon 1 play antagonistic roles in transcriptional regulation: bases from 1 to 20 strongly increase the transcription of the reporter gene CAT directed by the human TN-C gene promoter, while bases from 79 to 179 significantly reduce this activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules, Neuronal / genetics*
  • Chloramphenicol O-Acetyltransferase / genetics
  • Cricetinae
  • Enhancer Elements, Genetic / genetics*
  • Exons / genetics*
  • Extracellular Matrix Proteins / genetics*
  • Genes / genetics
  • Genes, Reporter / genetics
  • Humans
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic / genetics
  • RNA, Messenger / analysis
  • Sequence Homology, Nucleic Acid
  • Tenascin
  • Transcription, Genetic / genetics*
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Cell Adhesion Molecules, Neuronal
  • Extracellular Matrix Proteins
  • RNA, Messenger
  • Tenascin
  • Chloramphenicol O-Acetyltransferase