Abstract
Three genes encoding arylamine N-acetyltransferase were identified in Balb/c mice. All three genes were cloned from genomic DNA, sequenced and expressed in a bacterial expression system. Two of the genes corresponded to Nat-1 and Nat-2 which have been previously identified in A/J and C57B1/6 strains of mice (Martell et al., 1991). The new gene, designated Nat-3, can be distinguished from the other mouse Nat genes both by specific amplification using PCR and by restriction-endonuclease digestion. The products of all three genes are demonstrated to catalyse acetylation of aminofluorene and anisidine following expression in Escherichia coli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetylation
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Amino Acid Sequence
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Animals
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Arylamine N-Acetyltransferase / chemistry
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Arylamine N-Acetyltransferase / genetics*
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Arylamine N-Acetyltransferase / metabolism
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Base Sequence
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Blotting, Southern
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Cloning, Molecular
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DNA / chemistry
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DNA / genetics
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / genetics
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Isoenzymes / chemistry
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Isoenzymes / genetics*
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Isoenzymes / metabolism
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Mice
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Mice, Inbred A
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Molecular Sequence Data
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Polymerase Chain Reaction
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Restriction Mapping
Substances
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Isoenzymes
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Recombinant Proteins
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DNA
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Arylamine N-Acetyltransferase