Arylamine N-acetyltransferase in Balb/c mice: identification of a novel mouse isoenzyme by cloning and expression in vitro

Biochem J. 1994 Sep 1;302 ( Pt 2)(Pt 2):347-53. doi: 10.1042/bj3020347.

Abstract

Three genes encoding arylamine N-acetyltransferase were identified in Balb/c mice. All three genes were cloned from genomic DNA, sequenced and expressed in a bacterial expression system. Two of the genes corresponded to Nat-1 and Nat-2 which have been previously identified in A/J and C57B1/6 strains of mice (Martell et al., 1991). The new gene, designated Nat-3, can be distinguished from the other mouse Nat genes both by specific amplification using PCR and by restriction-endonuclease digestion. The products of all three genes are demonstrated to catalyse acetylation of aminofluorene and anisidine following expression in Escherichia coli.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylation
  • Amino Acid Sequence
  • Animals
  • Arylamine N-Acetyltransferase / chemistry
  • Arylamine N-Acetyltransferase / genetics*
  • Arylamine N-Acetyltransferase / metabolism
  • Base Sequence
  • Blotting, Southern
  • Cloning, Molecular
  • DNA / chemistry
  • DNA / genetics
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / genetics
  • Isoenzymes / chemistry
  • Isoenzymes / genetics*
  • Isoenzymes / metabolism
  • Mice
  • Mice, Inbred A
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / metabolism
  • Restriction Mapping

Substances

  • Isoenzymes
  • Recombinant Proteins
  • DNA
  • Arylamine N-Acetyltransferase

Associated data

  • GENBANK/X72959