Abstract
A fragment of the DNA polymerase I-encoding gene (polI) from Bacillus stearothermophilus (Bst) was obtained by PCR. This was used as a probe to obtain a full-length gene from a Bst genomic DNA (gDNA) plasmid library. Comparison of the sequence to B. caldotenax (Bca) showed about 93% homology at the amino acid (aa) level. A Klenow-like (BstpolIk) clone was developed and the recombinant protein displayed DNA polymerase activity similar to the wild-type BstPolI enzyme.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacillus / enzymology
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Bacillus / genetics
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Base Sequence
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Cloning, Molecular
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DNA Polymerase I / biosynthesis
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DNA Polymerase I / genetics*
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DNA Polymerase II / biosynthesis
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DNA Polymerase II / genetics
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Genes, Bacterial*
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Genome, Bacterial
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Genomic Library
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Geobacillus stearothermophilus / enzymology*
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Geobacillus stearothermophilus / genetics*
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Molecular Sequence Data
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Plasmids
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Polymerase Chain Reaction
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Restriction Mapping
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Sequence Homology, Amino Acid
Substances
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DNA Polymerase I
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DNA Polymerase II