Abstract
CD40 ligand (CD40L) is a member of the tumor necrosis factor superfamily and is expressed on the surface of activated T lymphocytes. The interaction of CD40L with CD40 on B cells results in B cell activation, immunoglobulin (Ig) secretion and Ig class switching. To study anergy as a mechanism of murine CD4 T cell tolerance, we determined both in vivo and in vitro that CD3-activated anergic cells are deficient in the ability to stimulate B cell proliferation, and that anergic cells are defective for the T cell receptor/CD3-mediated induction of CD40L expression. These results have implications for the recruitment of B cell responses by anergic T cells in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocytes / immunology
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CD4-Positive T-Lymphocytes / immunology*
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CD4-Positive T-Lymphocytes / metabolism
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CD40 Antigens / immunology
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CD40 Ligand
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Clonal Anergy*
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Female
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Gene Expression Regulation
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Lymphocyte Cooperation*
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Membrane Glycoproteins / biosynthesis*
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Membrane Glycoproteins / genetics
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Mice
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Mice, Inbred CBA
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Mice, Transgenic
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Receptor-CD3 Complex, Antigen, T-Cell / immunology
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Receptors, Antigen, T-Cell, alpha-beta / genetics
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Spleen / cytology
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Spleen / immunology
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T-Lymphocyte Subsets / immunology*
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T-Lymphocyte Subsets / metabolism
Substances
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CD40 Antigens
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Membrane Glycoproteins
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Receptor-CD3 Complex, Antigen, T-Cell
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Receptors, Antigen, T-Cell, alpha-beta
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CD40 Ligand