Molecular cloning of a widely expressed human homologue for the Drosophila trp gene

FEBS Lett. 1995 Oct 16;373(3):193-8. doi: 10.1016/0014-5793(95)01038-g.

Abstract

The Drosophila transient receptor potential (trp) gene and its homologue, trpl, have been suggested to mediate calcium entry during the insect's phototransduction process. We isolated a human cDNA, human trp-1 (Htrp-1), encoding a polypeptide of 793 amino acids that is 37% identical (62% similar) to Drosophila trp and trpl. Northern analysis showed that the Htrp-1 transcript is approximately 5.5 kb and expressed in most human tissues, with higher amounts in ovary, testis, heart, and brain. Isolation of Htrp-1 suggests that a trp-type protein is present in mammals and should provide a useful tool in studying calcium-depletion induced calcium influx processes.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Evolution
  • Blotting, Northern
  • Calcium / metabolism
  • Calcium Channels / chemistry
  • Calcium Channels / genetics*
  • Calmodulin-Binding Proteins / chemistry
  • Calmodulin-Binding Proteins / genetics
  • Cloning, Molecular*
  • DNA, Complementary
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Gene Expression
  • Genes, Insect
  • Humans
  • Inositol 1,4,5-Trisphosphate / metabolism
  • Insect Hormones / chemistry
  • Insect Hormones / genetics*
  • Insect Proteins*
  • Membrane Proteins / chemistry
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • TRPC Cation Channels
  • Transient Receptor Potential Channels

Substances

  • Calcium Channels
  • Calmodulin-Binding Proteins
  • DNA, Complementary
  • Drosophila Proteins
  • Insect Hormones
  • Insect Proteins
  • Membrane Proteins
  • TRPC Cation Channels
  • Transient Receptor Potential Channels
  • transient receptor potential cation channel, subfamily C, member 1
  • trp protein, Drosophila
  • trpl protein, Drosophila
  • Inositol 1,4,5-Trisphosphate
  • Calcium

Associated data

  • GENBANK/U31110