Identification of a new member of the human eIF-5A gene family

K Koettnitz; T Wöhl; B Kappel; F Lottspeich; J Hauber; D Bevec

doi:10.1016/0378-1119(95)00136-t

Identification of a new member of the human eIF-5A gene family

Gene. 1995 Jul 4;159(2):283-4. doi: 10.1016/0378-1119(95)00136-t.

Authors

K Koettnitz¹, T Wöhl, B Kappel, F Lottspeich, J Hauber, D Bevec

Affiliation

¹ SANDOZ Research Institute, Vienna, Austria.

PMID: 7622067
DOI: 10.1016/0378-1119(95)00136-t

Abstract

Using an oligodeoxynucleotide generated by rapid PCR amplification of 5'-cDNA ends (5'-RACE) as a detection probe, we have isolated a new genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A). Sequence analysis revealed that the eIF-5A coding region is identical to the corresponding cDNA but interrupted by three introns. In a plasmid shuffle experiment we show functional replacement of the essential homologous gene in Saccharomyces cerevisiae by this human eIF-5A.

MeSH terms

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA, Complementary / genetics
Eukaryotic Translation Initiation Factor 5A
Genetic Complementation Test
Humans
Molecular Sequence Data
Multigene Family / genetics*
Peptide Initiation Factors / classification
Peptide Initiation Factors / genetics*
Polymerase Chain Reaction
RNA-Binding Proteins*
Saccharomyces cerevisiae / genetics
Sequence Analysis, DNA
Sequence Homology, Amino Acid

Substances

DNA, Complementary
Peptide Initiation Factors
RNA-Binding Proteins

Associated data

GENBANK/U17969